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Generation Scotland Resources
Generation Scotland is creating a series of biomedical resources for the study of common complex disease in Scotland. To see the policy governing the use of GS data and biological samples, please visit the page Access to Generation Scotland Resources.
GS currently has three main collections of biological samples and data:
Resource GS:SFHS - The Scottish Family Health Study
Resource GS:21CGH - Genetic Health in the 21st Century
Resource GS:3D - Donor DNA Databank
Resources and Documentation
Resource GS:SFHS - The Scottish Family Health Study
GS:SFHS is an intensively phenotyped, family-based cohort for the study of the genetic basis of common complex diseases and response to treatments. Recruitment began early in 2006 and the cohort currently numbers around 6,500. Each participant gives blood samples (for DNA, serum and cryopreservation) and a urine sample.
Study methodology:
Please see the following reference for a description of the methods used:
Smith BH et al:
Generation Scotland: the Scottish Family Health Study; a new resource for researching genes and heritability
BMC Med Genet (2006) 7:74
Study documentation:
Participant Information Leaflet
Consent Form
Data overview:
1. Pre-clinic questionnaire
a. Demographic details
b. Occupational history
c. Lifestyle – smoking, alcohol, diet and exercise
d. Personal and family medical history
e. Rose angina questionnaire
f. Musculoskeletal questions – including Chronic Pain Grade, history of fracture and fragility fracture, personal family history of joint replacement, HRT use, age at menarche and menopause
g. Current drug history, including prescribed and non-prescribed medications
2. Physical measurements
a. Height, weight, waist-hip ratio
b. Blood pressure x 2 (Omron BP Monitor), resting pulse
c. Ankle brachial pressure index (ABPI)
d. Spirometry and FEVI
e. ECG
f. Bioimpedance
3. Cognitive function measurements
a. Eysenck Personality Questionnaire Revised Short Form
b. Logical Memory from the Wechsler Memory Scale III
c. Digit Symbol from the Wechsler Adult Intelligence Scale III
d. Verbal Fluency
e. Mill Hill Vocabulary Scale
f. General Health Questionnaire – 28
4. Mental health measurements
a. SCID Brief screening interview for major depression
Questionnaires:
Clinical Record Form
Pre-clinical Questionnaire
Target: up to approximately 50,000 family members
Resource GS:21CGH - Genetic Health in the 21st Century
GS:21CGH aims to establish the genetic profile of a control population of people living in Scotland in relation to health and disease. 21CGH is a growing resource of control DNA and genetic and phenotypic information. Recruitment began late in 2006 in Edinburgh, Aberdeen, Banff and Peterhead and the cohort currently numbers around 650. Two blood samples (for DNA, PBLs and plasma) are collected from each participant as well as a urine sample (Aberdeen only) and associated phenotype information including basic physical observations, answers to a clinical/lifestyle questionnaire, and cognitive function measurements. Data obtained from participants in the clinics is entered into the 21CGH database using electronic collection methods. Consent is also obtained for access to and subsequent extraction of de-identified information from patient medical records.
Study methodology:
DNA will be extracted from all blood samples and archive and working stocks prepared. Purified blood cells will be stored and used to make lymphoblastoid cell lines. These will primarily be a resource for proteomics work but they may also be used for DNA extraction for future research.
The 21CGH resource should limit the need to obtain further fresh control blood/DNA samples and will provide a knowledge base to aid in the design of future genetic studies.
Study documentation:
Participant Information Leaflet
Consent Form
Data overview:
Questionnaire
Target: approximately 2000 control subjects
Resource GS:3D - Donor DNA Databank
The Generation Scotland Donor DNA Databank (GS:3D) is building a large collection of human control DNA samples and plasma from blood donors attending sessions at the Scottish National Blood Transfusion Service throughout Scotland, together with associated basic phenotype data. Recruitment began early in 2008 and the cohort currently numbers over 4,900.
Study methodology:
The purpose of GS:3D is to provide a long term, well characterised resource of human DNA control samples. This could lead to an improvement in the statistical power of many human genetics studies and avoid duplication of effort and expense. 'Case' samples are unique to each disease study, but the same control samples can be used across many studies if appropriately collected and characterised. GS:3D provides access to a collection of well-maintained stocks of control DNA obtained with consent from the Scottish population. Donors will answer a short questionnaire and allow 10 ml of their blood and a filter that would otherwise be discarded to be used by the project. Questionnaire data is entered into the databank using optical mark read (OMR) technology. The collected data, DNA and plasma samples are irreversibly anonymised 28 days after collection.
Study documentation:
Participant Information Leaflet
Consent Form
Data overview:
Questionnaire
Age group, Sex
Cultural Background
Participant and Parental Scottish Region or Country of Origin
Grandparental Origin (Scotland/Other)
SNBTS donor inclusion criteria
Accessing GS Resources
For information about using the collections described here, please visit the page Access to Generation Scotland Resources.
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